2D-DIGE image of proteins of sarkosyl-insoluble aggregates isolated from the BY4742 [<i>PIN</i><sup>+</sup>] strain expressing PrP-GFP and from its [<i>pin</i><sup>−</sup>] derivative expressing non-fused GFP.

<p>Spots corresponding to proteins from [<i>PIN</i><sup>+</sup>] cells expressing PrP-GFP are red, while those from GFP-containing cells (control) are green. Yellow spots correspond to proteins present in both compared samples. Identification data are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone-0116003-t002" target="_blank">Table 2</a>; mass spectra are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone.0116003.s002" target="_blank">S2 Table</a>.</p>