2D-DIGE image of proteins of sarkosyl-insoluble aggregates isolated from the BY4742 [PIN+] strain expressing PrP-GFP and from its [pin] derivative expressing non-fused GFP.

Spots corresponding to proteins from [PIN+] cells expressing PrP-GFP are red, while those from GFP-containing cells (control) are green. Yellow spots correspond to proteins present in both compared samples. Identification data are presented in Table 2; mass spectra are shown in S2 Table.