2DNAGE of Bsp1407I- and BshNI-digested mtDNA from adults, with explanatory drawings.

Replication intermediates from adult flies digested with Bsp1407I and BshNI, hybridized with probe 6. Material was further digested with S1 nuclease (panels B) or was untreated (panels A). C) Summary drawing showing major replication intermediates inferred from the gels; ey – eyebrow arc, ss c – circles with variable ssDNA region, ez1 & 2– slow zones 1 & 2 on the eyebrow arc, other structures are labeled as in Figs 1 and 2. As in the case of the PstI digest, migration patterns of certain structures overlap before and after S1 treatment.