Yam storage protein dioscorins modulate cytokine gene expression in BALB/c and C57BL/6 lymphocytes

2015-10-08T14:52:14Z (GMT) by Yu-Jhen Hsu Jung-Hsin Hsu Kuo-Chih Lin
<div><p>This study aimed to evaluate the effects of dioscorins isolated from 2 yam species, <i>Dioscorea alata</i> (Da-dioscorins) and <i>Dioscorea japonica</i> (Dj-dioscorins), on mouse immune activities. Results from cytokine array indicated that Dj-dioscorins increased the production of tumor necrosis factor (TNF)-α, interleukin (IL)-2, IL-3, IL-6, IL-10, IL-12p40, IL-13, monocyte chemoattractant protein (MCP)-1, regulated on activation, normal T cell expressed and secreted (RANTES), and granulocyte colony-stimulating factor (GCSF) in the spleen cells of BALB/c mice. In RAW264.7 macrophage, Da-dioscorins upregulated the expression of TNF-α, IL-1β, IL-6, IL-10, RANTES, MCP-1, and GCSF genes to a greater extent than Dj-dioscorins did. TNF-α, IL-1β, IL-6, IL-10, and RANTES gene expression was higher in spleen cells than in bone marrow and thymus cells in Da-dioscorin- or Dj-dioscorin-treated BALB/c and C57BL/6. Overall, our results suggest that dioscorins exert distinct immunomodulatory effects on mouse immune cells, and that different mouse strains respond differently to dioscorins.</p></div>