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Western blots with (A) MAb Acetyl-K(103) and (B) MAb 2C3 loading control.

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posted on 2017-06-27, 17:32 authored by Deborah M. B. Post, Birgit Schilling, Lorri M. Reinders, Alexandria K. D’Souza, Margaret R. Ketterer, Steven J. Kiel, Aroon T. Chande, Michael A. Apicella, Bradford W. Gibson

Samples were taken from broth-grown cultures of 1291wt and 1291ackA at 3, 6, and 24h. The MAb Acetyl-K(103) blot shows that acetylation levels in 1291ackA are higher at every time-point compared to its parent strain 1291wt. The 2C3 blot confirms that equivalent levels of protein were used in these experiments.

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