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Western blot analysis of the expressed BRP, CAT and CSFVRP proteins.

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posted on 2017-11-01, 17:32 authored by Athanasios Niarchos, Anastasia Siora, Evangelia Konstantinou, Vasiliki Kalampoki, George Lagoumintzis, Konstantinos Poulas

BL21 E. coli transformed with recombinant pET-BccI/BRP or pET-BccI/CAT were lysed after protein expression and analyzed by Western blot. 1, 2, 3: Lysates from cultures inoculated with colonies transformed with pET-BccI/BRP 4, 5, 6: lysates from cultures inoculated with colonies transformed with pET-BccI/CAT and 7,8,9: lysates from cultures inoculated with colonies transformed with pET-BccI/ CSFVRP.

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