Video recording of freely diffusing 60 nm and 80 nm gold nanoparticles using dark field microscopy
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Dark field recordings of 60 nm and 80nm gold particles which were diluted in essentially particle-free double distilled H2O. The temperature was 24 °C. The concentration was amounted to approximately 3x10^9 ml.
The video file was captured with a Canon EOS 5D Mark II camera at 25 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser.
The video pixel size is 182 nm.
This video is part of a puplication:
Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x