Additional_File_12_Video_7.mov (65.12 MB)
Video 7: Rapid volumetric calcium imaging of highly motile Hydra.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerGCaMP6s expressing Hydra was imaged using LSTM with 10x/0.6NA objective. Maximum intensity projections are shown for the two halves of the volume. First occurrences of longitudinal and radial contractions are annotated. The scale Bar is 100 microns.
An advantage of the LSTM imaging technique (see below for details) is the capacity for rapid volumetric live imaging of samples undergoing substantial non-isomorphic rearrangements in their body shape and cellular density, resulting in continuously changing local optical properties.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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