Additional_File_10_Video_5.mov (140.89 MB)
Video 5: Visualization of an image stack of vasculature stained rat brain tissue.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerThis video visualizes an image stack acquired from a large rat brain slice (stained for vasculature with tomato lectin) using LSTM in 2-AS (2-axes scan) mode. The bounding box is 1mm x 1mm x 5mm. Uniform high-resolution imaging of the entire tissue under LSTM is presented.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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