figshare
Browse
Additional_File_9_Video_4.mov (164.94 MB)

Video 4: High-resolution LSTM imaging of a large tissue of Thy1-eYFP mouse brain.

Download (164.94 MB)
media
posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju Tomer
The bounding box is 9.6mm x 13.5 mm x 5.34 mm. The raw data was down-sampled 4x4 fold to make the volume rendering feasible.

Imaging presents a thick coronal slice (~9.6mm x 13.5 mm x 5.34 mm; the sample expanded ~1.5-2 fold due to the immersion in glycerol solution) of a CLARITY-cleared Thy1-eYFP transgenic mouse brain, with 10x/0.6NA/8mmWD.

This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.

LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.

View the full collection of related videos at:

https://doi.org/10.6084/m9.figshare.c.4072160

Funding

This work is mainly supported by Columbia University Arts & Sciences startup grant to RT. Hermanson445 Lab and BM are supported by VR-MH and BCF. RY and CD were supported by the NEI (DP1EY024503),446 and the reported Hydra material is based upon the work supported by the Defense Advanced Research447 Projects Agency (DARPA) under Contract No. HR0011-17-C-0026. In addition, Hydra research was448 supported in part by competitive fellowship funds from the H. Keffer Hartline, Edward F. MacNichol, Jr.449 Fellowship Fund, The E. E. Just Endowed Research Fellowship Fund, Lucy B. Lemann Fellowship Fund,450 and Frank R. Lillie Fellowship Fund Fellowship Fund of the Marine Biological Laboratory in Woods Hole,451 MA.

History

Research Data Support

Research data support provided by Springer Nature.