Additional_File_9_Video_4.mov (164.94 MB)
Video 4: High-resolution LSTM imaging of a large tissue of Thy1-eYFP mouse brain.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerThe bounding box is 9.6mm x 13.5 mm x 5.34 mm. The raw data was down-sampled 4x4 fold to make the volume rendering feasible.
Imaging presents a thick coronal slice (~9.6mm x 13.5 mm x 5.34 mm; the sample expanded ~1.5-2 fold due to the immersion in glycerol solution) of a CLARITY-cleared Thy1-eYFP transgenic mouse brain, with 10x/0.6NA/8mmWD.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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