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VesB association with OMVs.

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posted on 2018-10-23, 17:38 authored by Shilpa Gadwal, Tanya L. Johnson, Henriette Remmer, Maria Sandkvist

Supernatants containing VesB, VesBΔ5 and VesBΔ30 were centrifuged at 200,000 x g for 3 hours to pellet OMVs. Supernatant (s), cleared supernatant (cs) and resuspended outer membrane vesicles (omv) were analyzed by SDS-PAGE and immunoblotting with antibodies recognizing VesB and the outer membrane protein OmpU.

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