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Versatile Microfluidic Droplets Array for Bioanalysis
journal contribution
posted on 2015-01-14, 00:00 authored by Shan-Wen Hu, Bi-Yi Xu, Wei-ke Ye, Xing-Hua Xia, Hong-Yuan Chen, Jing-Juan XuWe propose a novel method to obtain
versatile droplets arrays on
a regional hydrophilic chip that is fabricated by PDMS soft lithography
and regional plasma treatment. It enables rapid liquid dispensation
and droplets array formation just making the chip surface in contact
with solution. By combining this chip with a special Christmas Tree
structure, the droplets array with concentrations in gradient is generated.
It possesses the greatly improved performance of convenience and versatility
in bioscreening and biosensing. For example, high throughput condition
screening of toxic tests of CdSe quantum dots on HL-60 cells are conducted
and cell death rates are successfully counted quickly and efficiently.
Furthermore, a rapid biosensing approach for cancer biomarkers carcinoma
embryonic antigen (CEA) is developed via magnetic beads (MBs)-based
sandwich immunoassay methods.