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Verification of DEGs using qRT-PCR.

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posted on 2019-04-17, 17:39 authored by Zhou-Xiang Liao, Zhe Ni, Xin-Li Wei, Long Chen, Jian-Yuan Li, Yan-Hua Yu, Wei Jiang, Bo-Le Jiang, Yong-Qiang He, Sheng Huang

(A)The line shows the differences in the expression levels resulting from RNA-seq in rice leaves infected with the GX01 T3SD and GX01 wild-type (WT) strains represented as log2(FPKMT3SD/FPKMWT). The columns show expression level differences based on qRT-PCR assay results (three biological replicates). (B) The line shows the differences in the expression levels resulting from RNA-seq and displays the fold changes of GX01 growing in planta compared to in media represented as log2(FPKM in planta/FPKM in media). The columns show expression level differences in the qRT-PCR assay results (three biological replicates). All qPCR data were processed using the 2(-ΔΔC(t)) method.

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