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Vascular effects of R-568 in MVB from WKY and SHR rats.

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posted on 2018-08-09, 17:58 authored by Natalia Di Pietro, Maria Assunta Potenza, Sara Di Silvestre, Francesco Addabbo, Nadia Di Pietrantonio, Pamela Di Tomo, Caterina Pipino, Domitilla Mandatori, Carola Palmerini, Paola Failli, Mario Bonomini, Monica Montagnani, Assunta Pandolfi

Mesenteric vascular beds (MVB) from 8-week old WKY and SHR rats were isolated and prepared as described in Methods. PP at approximately 120 mmHg was maintained by continuous infusion with NA (10 μM and 3 μM in WKY and SHR rats, respectively). (A) and (B) Representative tracings of vasodilator responses to submaximal ACh dose (1 μM) and to increasing concentrations of R-568 (10 nM—30 μM) in WKY (A) and SHR (B) are shown. Each symbol represents the start of a 4-min perfusion. (C) Dose-response curves for R-568-induced vascular effects were obtained from MVB of WKY and SHR. Results are the mean ± SEM of 7 (WKY) and 6 (SHR) independent experiments. Data from each curve were normalized by defining 100% as the initial steady state PP and 0% as the maximal reduction in PP obtained in WKY treated with 1 μM ACh. *p < 0.01 vs respective values.

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