Validation of the regulatory activities of FMN, TPP riboswitches and a putative 5’-UTR regulatory candidate in different nutrient conditions.

<p>The relative expression and average RNA-Seq coverage of SP_0178 (FMN) <b>(A)</b> and SP_0716 (TPP) <b>(C)</b> increases in poor (MCDM) medium compared to rich (SDMM) medium, potentially compensating for the depletion of the specific ligand. Error bars represent standard error of the mean. Expression of SP_0178 (FMN) <b>(B)</b> and SP_0716 (TPP) <b>(D)</b> is reduced when the poor medium is supplemented with respective ligands thus confirming the regulatory activities of FMN and TPP riboswitches. (FMN- Riboflavin; TPP- Thiamine). Expression is relative to the qPCR internal control and thus for instance an expression level of 0.5 means that the expression of tested gene is 50% of the expression of internal control. Error bars represent standard error of the mean across three technical replicates. SP_1782 encoding ribosomal protein L11 methyltransferase <b>(E)</b> decreases in poor media (MCDM) compared to rich media (SDMM).</p>