Fig 3.tif (164.43 kB)
Validation of microarray data on real time PCR by analyzing the relative expression of 5 genes in smear positive PTB samples as compared to in vitro grown M.tb H37Rv.
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posted on 2017-03-10, 18:37 authored by Sumedha Sharma, Michelle B. Ryndak, Ashutosh N. Aggarwal, Rakesh Yadav, Sunil Sethi, Shet Masih, Suman Laal, Indu Verma16s rRNA was used as reference gene for normalization. Y-axis values (Log2 fold change) of ≥1 indicate upregulation and values ≤ -1 indicate down-regulation. Each bar represents mean ± SD values for each of the genes with three technical replicates. *p<0.05; **p<0.01 by student’s t test.
