Validation of microarray data on real time PCR by analyzing the relative expression of 5 genes in smear positive PTB samples as compared to <i>in vitro</i> grown <i>M</i>.<i>tb</i> H37Rv.

<p>16s rRNA was used as reference gene for normalization. Y-axis values (Log2 fold change) of ≥1 indicate upregulation and values ≤ -1 indicate down-regulation. Each bar represents mean ± SD values for each of the genes with three technical replicates. *p<0.05; **p<0.01 by student’s t test.</p>