jf6b01817_si_001.pdf (18.01 MB)
Using PacBio Long-Read High-Throughput Microbial Gene Amplicon Sequencing To Evaluate Infant Formula Safety
journal contribution
posted on 2016-08-08, 00:00 authored by Yi Zheng, Xiaoxia Xi, Haiyan Xu, Qiangchuan Hou, Yanfei Bian, Zhongjie Yu, Lai-Yu Kwok, Wenyi Zhang, Zhihong Sun, Heping ZhangInfant
formula (IF) requires a strict microbiological standard
because of the high vulnerability of infants to foodborne diseases.
The current study used the PacBio single-molecule real-time (SMRT)
sequencing platform to generate full-length 16S rRNA-based bacterial
microbiota profiles of 30 Chinese domestic and imported IF samples.
A total of 600 species were identified, dominated by Streptococcus thermophilus, Lactococcus
lactis, and Lactococcus piscium. Distinctive bacterial profiles were observed between the two sample
groups, as confirmed with both principal coordinate analysis and multivariate
analysis of variance. Moreover, the product whey protein nitrogen
index (WPNI), representing the degree of preheating, negatively correlated
with the relative abundances of the Bacillus genus. This study has demonstrated the application of the PacBio
SMRT sequencing platform in assessing the bacterial contamination
of IF products, which is of interest to the dairy industry for effective
monitoring of microbial quality and safety during production.
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Keywords
microbiota profilesLactococcus lactis16 S rRNA-basedStreptococcus thermophilussample groupsWPNIFormula SafetyBacillus genusfoodborne diseasesPacBio SMRT sequencing platform600 speciesLactococcus pisciummultivariate analysisPacBio Long-Read High-Throughput Microbial Gene Amplicon Sequencingsequencing platformPacBio single-moleculeproduct whey protein nitrogen indexdairy industry
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