Ultrastructure of the SH-SY5Y cell line cultivated in absence and presence of Pt-Diss.

After cultivation either without any Pt as reference (A) or in culture medium containing 6.0 μg/ml (B), 0.11 μg/ml (C) or 0.02 μg/ml Pt (D) SH-SY5Y were characterized by large euchromatic nucleus, abundant endoplasmic reticulum and a few synaptic granules (arrowheads). At the highest tested Pt concentration these cells were adversely affected, as proven by mitochondrial swelling (arrow in B). A smaller amount of Pt in the culture medium induced no morphological changes in comparison with the control. Size of bars: 2 μm.