Two types of pSNMs revealed by inter- and intra-organ profiles.

(A) Minor allele fractions between different categories of pSNM. The organ-shared pSNMs demonstrated significantly higher allele fractions than the organ-unique pSNMs. (B) Number of pSNMs carried in different organ samples. Red and blue bars denote the organ-shared and organ-unique pSNMs, respectively. An excess of organ-unique pSNMs was observed in the breast sample of BBL11121 and the liver sample of BBLD1005. (C-D) Heatmap of minor allele fractions for pSNMs carried in multiple organ samples of BBLD1005 (C) and BBL11121 (D). Blood samples of two unrelated individuals (ACC1 and ACC4) served as negative controls. The color intensity of each tile represents allele fractions estimated by targeted ultra-depth resequencing. Gray tiles denote sites without sufficient read depth (< 30X). Red and blue bars denote the organ-shared and organ-unique pSNMs, respectively. The majority of organ-unique pSNMs were locally restricted to one or a few physically adjacent organ samples (<1 cm). (E-F) Relative intra-organ similarity of pSNM profiles in BBLD1005 (E) and BBL11121 (F). The originally sequenced samples (liver #9 and breast #9) shared the largest similarity to their physically closest samples.