TIFF series of diSPIM datasets, fused and deconvolved, from A549 GFP-Rab11A cells infected with WSN H1N1 PA::mRuby influenza virus. Each TIFF file is a full cell volume of 50 planes, with isotropic resolution of 330nm, each .zip file is an independent replicate with at least 1 or 2 cells analyzed for 3D tracking of cytoplasmic foci with over 100 time stamps. These data correspond to Supplementary Movie 11 and analysis of these movies provided data presented in Figure 6 and Supplementary Figure 11.
Supplementary Movie 11: A549 GFP-Rab11A infected with PA::mRuby virus.
Figure 6: Two-color imaging of PA::mRuby infected GFP-Rab11A A549 cells.
Supplementary Figure 11: Additional motion parameters for two-color imaging datasets.
Funding
Visualization of Influenza Viral RNA Assembly
National Institute of Allergy and Infectious Diseases