Tupichinins B–D, three new spirostanol saponins from <i>Tupistra chinensis</i> rhizomes

<div><p>Three new spirostanol saponins were isolated from the EtOAc fraction of methanol extract from <i>Tupistra chinensis</i> rhizomes. Based on the detailed analysis of their 1D and 2D NMR spectra and chemical evidence, their structures were determined as 1β-<i>O</i>-acetyl-spirost-5,25(27)-dien-3α-yl-<i>O</i>-β-d-glucopyranoside (<b>1</b>), (25<i>S</i>)-1β,2β,5β-trihydroxy-spirostane-3β-yl-<i>O</i>-β-d-glucopyranoside (<b>2</b>) and (25<i>S</i>)-1β,2β-dihydroxy-5β-spirostane-3β-yl-<i>O</i>-β-d-xylopyranoside (<b>3</b>), respectively.</p></div>