Trichostatin A suppressed lung stretch-induced collagen gene expression.
After five days of bleomycin treatment, real-time polymerase chain reaction of fibronectin (A), MMP-9 (B), and 1a1 (C) mRNA expression were from the lungs of nonventilated control mice and mice ventilated at a tidal volume of 6 mL/kg or 30 mL/kg for 5 h with room air (n = 5 per group). Arbitrary units were expressed as the ratio of fibronectin, MMP-9, and 1a1 mRNA to GAPDH (n = 5 per group). TSA 2 mg/kg was given intraperitoneally 1 h before mechanical ventilation. ANOVA was used to assess the statistical significance of the differences, followed by multiple comparisons with a Scheffeˊs test, and a P value < 0.05 was considered statistically significant. * P< 0.05 versus the nonventilated control mice with bleomycin pretreatment; † P< 0.05 versus all other groups.