Toxicological evaluation of asparaginase purified from <i>E</i>. <i>cloacae</i>.

<p><b>(a)</b> Non-toxic effects of purified asparaginase on noncancerous human epithelial cell line FR-2. Approximately, 1.5x10<sup>4</sup> cells ml<sup>-1</sup> were cultured at 37°C and treated with 2, 5, 10, and 15 IU ml<sup>-1</sup> concentrations of purified asparaginasefor 48 h and cell viability was determined by the MTT assay. <b>(b(i)</b> Crude asparaginase formed clear translucent zone on blood agar plate due to lysis of erythrocytes. <b>(b(ii)</b> Purified asparaginase had no hemolytic effect. <b>(c)</b> Quantitative measure of the haemolytic activity. A: negative control (without asparaginase); B: positive control (Phosphate buffer); C: crude asparaginase; D: 15 IU ml<sup>-1</sup>; E: 7.5 IU ml<sup>-1</sup>; F: 3.75 IU ml<sup>-1</sup> concentrations of asparaginase.</p>