Tobacco Seed Growth in D2O

In 1950 Crumley et al did a series of experiments where they tested the growth rate of several plants in differing amounts of D2O. They used H2O, 33% D2O, 63% D2O, and 99% D2O (mixtures with H2O) and discovered that growth rates were drastically slower in increasing amounts of D2O. I was asked to repeat their experiment with just tobacco seeds and for the most part I validated their claims with one crucial exception: tobacco seeds in 99% D2O don't grow at all! All experimental information is kept in an open notebook online at and you can access all the trials for this experiment through this link: Contained on that page are links to an experimental summary, and links to each individual trial which will allow you access to see the setup and day to day results of each trial. Feel free to browse around and provide feedback.