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Tj controls the morphogenetic behavior and specification of cap cells.

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posted on 2017-05-19, 17:25 authored by Trupti Panchal, Xi Chen, Ekaterina Alchits, Youjin Oh, James Poon, Jane Kouptsova, Frank A. Laski, Dorothea Godt

Yellow arrowheads mark the germarium/TF boundary. (A-G) GSC niches of adult ovaries. LamC identifies TF and cap cells. In the control (wt) (A,C,E), cap cells form a cluster inside the germarium and TF cells form a stalk outside the germarium. In ovaries of tj39/tjeo2 (tjhypo) (B) and tjnull mutants (tjeo2/tjeo2 in D, tjeo2/tjDf1 in F,G), the cluster is missing and the stalk is abnormally long. (B) In a tjhypo ovary, the basal cells of the elongated stalk (between arrow and arrowhead) express markers in a cap cell-specific manner (strong Bab2 and 1444-lacZ signals), in contrast to distal cells in the stalk. (D,F,G) In tjnull ovaries, all cells in the elongated stalks express markers similar to normal TFs (weak and strong expression of Bab2 (D,F) and LB27-lacZ (F,G), respectively, and no detection of 1444-lacZ (D). (H,I) Adult GSC niches with tjz4735 mutant cell clones. tjz4735 mutant cap cells, which only express the non-functional isoform of Tj (red) and lack GFP (green), are located in the TF (above the arrowhead) and display the flat shape of TF cells (asterisks), whereas control cap cells, which co-express Tj and GFP, are located in the germarium (below the arrowhead). (J-M) Prepupal ovaries. TFs are longer in tjnull ovaries (tjeo2/tjeo2 in K, tjz4735/tjeo2 in M) than in control ovaries (J,L). (J,K) B1-lacZ (red) labels cap and TF cells. LamC (green), which is only seen in the distal half of TFs in the control ovary, is present in all B1-lacZ-positive cells of the tjnull ovary. The germ cell population, marked by Vasa (blue), is not divided into ovarioles in the tjnull ovary in contrast to the control. (L,M) 1444-lacZ (red), which labels nascent cap cells in the control ovary is not detected in the tjnull ovary. Tj (blue) is seen in cap cells but not in TFs in the control ovary. In the tjnull ovary, which expresses a mutant Tj isoform, Tj is seen in the basal portion of the elongated TFs (between arrowhead and arrow). Genotypic markers: 1444-lacZ/+ (A,B,L,M) or 1444-lacZ (C,D), LB27-lacZ/+ (E-G), Ubi-GFP (H,I), and B1-lacZ (J,K). Anterior is up. Scale bars: 10 μm in A-F,H-M; 50 μm in G.

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