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Three-Dimensional High-Resolution Rotational Tracking with Superlocalization Reveals Conformations of Surface-Bound Anisotropic Nanoparticles
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posted on 2016-02-19, 18:43 authored by Kyle Marchuk, Ji Won Ha, Ning FangThe ability to directly follow three-dimensional rotational
movement
of anisotropic nanoparticles will greatly enhance our understanding
of the way nanoparticles interact with surfaces. Herein, we demonstrate
dual-color total internal reflection scattering microscopy as a tool
to probe the interactions of plasmonic gold nanorods with functional
surfaces. By taking advantage of both the short and long axis surface
plasmon resonance scattering enhancement, we are able to decipher
both in-plane and out-of-plane gold nanorod motion relative to the
sample surface with equally high resolution. In combination with superlocalization
through point spread function fitting, we overcome the four-quadrant
angular degeneracy of gold nanorods in the focal plane of the objective
and resolve conformations of surface-bound anisotropic nanoparticles
in unprecedented detail.