The targeted U residue is required for stable sRNP/RNA-S complex formation

<p><b>Copyright information:</b></p><p>Taken from "Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation"</p><p>Nucleic Acids Research 2005;33(10):3133-3144.</p><p>Published online 2 Jun 2005</p><p>PMCID:PMC1142404.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Incubations was as in , lane 9, except that the WT (RNA-S) or mutated (RNA-S-mtU) target RNA was used at concentrations varying from 22 to 530 nM (molar excess from 3 to 48, as indicated above the lanes). The percentages of Pab91 sRNA in RNP5 and complex CII are given below the lanes.