The specific lytic activity of MCF-7 cancer stem-like cell antigens.

<p>(A) Identification of populations of MCF-7 cells by flow cytometry analysis of CD44 and CD24 expression. (B) Western blot showing CD44 and ALDH protein expression in CD44<sup>+</sup>, CD44<sup>-</sup> and CD44<sup>+</sup>CD24<sup>-</sup> MCF7- cells. (C) Targeted killing of CD44<sup>+</sup> MCF-7 cells by tumor-associated antigen-pulsed, DC-induced CTLs. The data are expressed as the mean±SD, and significant differences between the treated groups were detected using ANOVA followed by the Dunnett’s test. <sup>#</sup> <i>p</i><0.05 compared with the DC-CTLs. * <i>p</i><0.05 compared with the TL-CTLs.</p>