Figure_S1.jpeg (335.07 kB)
Figure S1 - The rnb Gene of Synechocystis PCC6803 Encodes a RNA Hydrolase Displaying RNase II and Not RNase R Enzymatic Properties
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posted on 2012-03-05, 02:13 authored by Rute G. Matos, Arsénio M. Fialho, Mordechai Giloh, Gadi Schuster, Cecília M. ArraianoExoribonucleolytic activity at 30°C of Synechocystis protein: comparison with E. coli RNase II and RNase R. Activity assays were performed using the three synthetic substrates: 30-mer poly(A), the 16-mer and the double-stranded substrate 16–30 ds. The concentration of proteins used is indicated in the figure. Samples were taken during the reaction at the time points indicated. Control reactions with no enzyme added (Ctrl) were incubated at the maximum reaction time for each protein. Length of substrates and degradation products are labelled.
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