figshare
Browse
Figure_S1.jpeg (335.07 kB)

Figure S1 - The rnb Gene of Synechocystis PCC6803 Encodes a RNA Hydrolase Displaying RNase II and Not RNase R Enzymatic Properties

Download (0 kB)
figure
posted on 2012-03-05, 02:13 authored by Rute G. Matos, Arsénio M. Fialho, Mordechai Giloh, Gadi Schuster, Cecília M. Arraiano

Exoribonucleolytic activity at 30°C of Synechocystis protein: comparison with E. coli RNase II and RNase R. Activity assays were performed using the three synthetic substrates: 30-mer poly(A), the 16-mer and the double-stranded substrate 16–30 ds. The concentration of proteins used is indicated in the figure. Samples were taken during the reaction at the time points indicated. Control reactions with no enzyme added (Ctrl) were incubated at the maximum reaction time for each protein. Length of substrates and degradation products are labelled.

(JPG)

History

Usage metrics

    PLOS ONE

    Categories

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC