The process of HDAC11 Assay Development: linearity and Km calculation
With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.
Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).
2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.