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The immunomodulatory effects of Zn-incorporated micro/nanostructured coating in inducing osteogenesis

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journal contribution
posted on 2018-03-08, 13:31 authored by Ranran Zhang, Xujie Liu, Zhiyuan Xiong, Qianli Huang, Xing Yang, Hao Yan, Jing Ma, Qingling Feng, Zhijian Shen

Micro/nanostructured TiO2/ZnO coating has been shown to possess multiple functions, including antibacterial activity and bioactivity. Osteoblast-like SaOS-2 cells were employed for evaluating the in vitro osteogenic capacity of this coating and positive results were obtained. However, traditional principles of osseointegration focus only on the osteogenic differentiation alone. The effects of immunomodulation on the osteogenic activity have been largely ignored. In this study, the inflammatory responses of macrophages on the micro/nanostructured TiO2/ZnO coating were investigated. The extract media of macrophage cell line RAW264.7 cultured on the TiO2/ZnO coating were collected as indirect co-culture conditioned media. The osteogenic activity of SaOS-2 cells in the conditioned media was investigated. Adhesion, ALP activity and extracellular mineralization of cells grown in the conditioned media extracted from the micro/nanostructured TiO2/ZnO coating were found to be enhanced, compared to those grown in the conditioned media extracted from the macroporous TiO2 coating. The immune microenvironment produced by the micro/nanostructured TiO2/ZnO coating showed excellent capacity to promote osteogenesis, indicating that this coating could be a promising candidate for implant surface modification in orthopaedic and dental applications. Furthermore, this work could help us understand the interplay between the host immune system and the osteoimmunomodulatory properties of the biomaterials, and optimize the design for coating biomaterials.

Funding

The authors are grateful for the financial support from National Natural Science Foundation of China [grant numbers U1605243, 51472139, 31600763], and International S&T Cooperation of the Ministry of Science and Technology of the People's Republic of China [grant number 2015DFG52490].

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