The Neurog3 enhancer region contributes to transcriptional repression in alternative lineages.

qRT-PCR analysis showed that in E13.5 liver progenitors and embryonic fibroblasts from transgenics carrying the Neurog3 enhancer, GFP mRNA was significantly reduced. GFP transcript levels were normalized for Tbp and compared to the construct that lacked the Neurog3 enhancer region ([*] (P<0.05, Student’s t-test).