The function of BCL9 in Wnt/β-catenin signaling and colorectal cancer cells-5

numbers in the latter two refer to mouse or human HD2 sequences, which are identical). Individual conserved residues () were mutated, and semiquantitative estimates of their binding affinities (++/+/-) to the β-catenin ARD are given underneath. , pull-down assays between translated wt and mutant BCL9 HD2 and GST-ARD, as indicated; input lanes, 5% of the total binding reaction. , co-immunoprecipitations of FLAG-tagged wt and mutant BCL9 or BCL9-2 with HA-β-catenin expressed in HEK 293T cells; , protein expression levels in the lysates.<p><b>Copyright information:</b></p><p>Taken from "The function of BCL9 in Wnt/β-catenin signaling and colorectal cancer cells"</p><p>http://www.biomedcentral.com/1471-2407/8/199</p><p>BMC Cancer 2008;8():199-199.</p><p>Published online 15 Jul 2008</p><p>PMCID:PMC2478683.</p><p></p>