pntd.0006120.g005.tif (1.34 MB)
The effects of ESPs on IEC cell signaling.
figure
posted on 2017-12-11, 18:41 authored by Showgy Y. Ma’ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G. Svärd(A) Western blot analyses of differentiated Caco-2 cells exposed to G. intestinalis ESPs through a Transwell insert for 2h or 6h in the absence of inflammatory stimuli (e.g. lipopolysaccharides or tumour necrosis factor-alpha). Blots were probed for total proteins (phosphorylated/non-phosphorylated forms) of mitogen activated proteins kinases (MAPKs, ERK1/2 and P38) and nuclear factor kappa beta (NFκB). (B) Western blot analyses after the induction of inflammation with LPS and TNF-alpha.
