The effect of immune modulation on RAW 264.7 NF-ĸB p65 expression when infected with Bp82 after 90 minutes of infection.

Cells were pretreated with 100 μM TA or 0.01% DMSO for 30 minutes along with an uninfected and untreated control. NF-ĸB p65 levels were determined flow cytometry using the Mean Channel Fluorescence (MCF) over 5,000 events from intact non-aggregated cells (see S1 Fig). Significance was determined by comparing to the vehicle control, where TA treatment was able to suppress p65 expression significantly. Data is representative of three biological replicates run in duplicate. Significance was determined via 2-way ANOVA with Bonferroni’s post-hoc test for significance. Error indicates SEM. ***p<0.001, ****p<0.0001.