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The effect of immune modulation on RAW 264.7 NF-ĸB p65 expression when infected with Bp82 after 90 minutes of infection.
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posted on 2016-10-28, 17:37 authored by William J. Wilson, Maryam F. Afzali, Jason E. Cummings, Marie E. Legare, Ronald B. Tjalkens, Christopher P. Allen, Richard A. Slayden, William H. HannemanCells were pretreated with 100 μM TA or 0.01% DMSO for 30 minutes along with an uninfected and untreated control. NF-ĸB p65 levels were determined flow cytometry using the Mean Channel Fluorescence (MCF) over 5,000 events from intact non-aggregated cells (see S1 Fig). Significance was determined by comparing to the vehicle control, where TA treatment was able to suppress p65 expression significantly. Data is representative of three biological replicates run in duplicate. Significance was determined via 2-way ANOVA with Bonferroni’s post-hoc test for significance. Error indicates SEM. ***p<0.001, ****p<0.0001.
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RAW 264.7 cellsCOX -2 inhibitionsurvival outcomeNF -ĸB activationCOX -2 inhibitoranimal useRecent studiespseudomallei MelioidosisUnited StatesTolfenamic acidPGE 2 productionB pseudomalleifatality ratefacultative intracellular bacterium Burkholderia pseudomalleiNF -ĸB protein expressioncyclooxygenase -2prostaglandin E 2post-exposure treatmentNSAIDBALBCZorgan tissuecell viabilitypost-exposure TA treatmentnon-steroidal anti-inflammatory drugCOX -2alternative COX -2 inhibitorImmune ModulationCOX -2 expressioncell survivalEffective Adjunct Post-exposure Therapeutic
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