ppat.1006829.g002.tif (642.05 kB)
The cGAS/STING axis contributes to the production of pro-inflammatory cytokines during L. pneumophila infection.
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posted on 2018-01-03, 18:40 authored by Juan S. Ruiz-Moreno, Lutz Hamann, Javeed A. Shah, Annelies Verbon, Frank P. Mockenhaupt, Monika Puzianowska-Kuznicka, Jan Naujoks, Leif E. Sander, Martin Witzenrath, John C. Cambier, Norbert Suttorp, Ralf R. Schumann, Lei Jin, Thomas R. Hawn, Bastian Opitz(A-F) WT, Tmem173-/- and cGas-/- BMDMs were infected for 6 h with L. pneumophila WT at MOI 10 and relative cytokine expression was determined by qRT-PCR. (G-J) Cytokine protein concentrations in whole lung homogenates from L. pneumophila-infected mice were quantified by sandwich ELISA. Data are shown as mean ± SEM. (A-F) Data representative of 3 to 4 independent experiments carried out in duplicates. (G-J) Data representative of 6 o 7 mice per group. Data were analyzed through the Mann-Whitney U Test. Comparisons with a p < 0.05 were considered significant.
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R 232H attenuated cytokine productionsusceptibilitySTING-deficient murine macrophagesR 232H allelescyclic GMP-AMP synthaseSTING-deficient animalscyclic dinucleotidesTMEMIFNprotozoal infectionsRecent studiesHAQ STING variant impairs cGAS-dependentcGAS-STING cascadeLegionnairehaplotype frequencyDNATrial registration ClinicalTrials.gov DRKS 00005274CDNmouse modelwild-type miceLegionella pneumophilaresponsepro-inflammatory cytokines
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