The ERK inhibition effect with AZD6244 was significant in neoplastic cells and mild in non-neoplastic cells.

(A).Representative immunoblots using antibodies against phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 (ERK1/2) to probe proteins extracted from MCF7, Colo205, 786-O, and Panc02 cell lysates treated with 50 nM, 100 nM, 200 nM, 500 nM, and 1μM AZD or without AZD, n = 3. (B).Representative immunoblots using antibodies against phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 (ERK1/2) to probe proteins extracted from BRL-3A, HUVEC, fibroblasts, and H9C2 cell lysates treated with50 nM, 100 nM, 200 nM, 500 nM, and 1μM AZD or without AZD, n = 3.