Temporal dynamics of T-bethi expression and effector HIV-specific CD4+ T cell responses following HIV infection in blood.
(A) Frequencies of T-bethi CD4+ T cells before and first sample taken 1 year after HIV infection (n = 10). (B) Longitudinal changes of T-bethi CD4+ T cells before and subsequently after HIV infection. Every individual is depicted with black connecting lines and red line indicate the estimated mean value (linear regression) over time (n = 10). (C) Frequency of T-bethi expression on memory CD4+ T cells in HIV- and HIV+ chronic progressors (CP) (D) T-bet, (E) perforin and (F) MIP-1α expression by IFNγ+ Gag-specific CD4+ T cells before and subsequently following HIV infection (n = 10). The colored lines represent each subject and their frequencies of T-bethi, perforin+ and MIP-1α+ Gag-specific CD4+ T cells over time. A Wilcoxon or Mann-Whitney test was performed to compare the difference between groups; *P < 0.05. Longitudinal data-points are derived from the RV217 cohort and cross-sectional data from the European cohort.
