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Targeted PRINT Hydrogels: The Role of Nanoparticle Size and Ligand Density on Cell Association, Biodistribution, and Tumor Accumulation
journal contribution
posted on 2015-10-14, 00:00 authored by Kevin G. Reuter, Jillian
L. Perry, Dongwook Kim, J. Christopher Luft, Rihe Liu, Joseph M. DeSimoneIn this Letter, we varied targeting
ligand density of an EGFR binding affibody on the surface of two different
hydrogel PRINT nanoparticles (80 nm × 320 and 55 nm × 60
nm) and monitored effects on target-cell association, off-target phagocytic
uptake, biodistribution, and tumor accumulation. Interestingly, variations
in ligand density only significantly altered in vitro internalization rates for the 80 nm × 320 nm particle. However, in vivo, both particle sizes experienced significant changes
in biodistribution and pharmacokinetics as a function of ligand density.
Overall, nanoparticle size and passive accumulation were the dominant
factors eliciting tumor sequestration.