TRPA1 and TRPV1 contribute to propofol-mediated antagonism of U46619-induced constriction in murine coronary arteries - Fig 4

Representative calcium imaging traces depicting the effect of single application of (A) propofol (prop; 10 μM), a TRPA1 agonist; (B) propofol in the presence of HC-030031 (1 μM), a TRPA1 antagonist; (C) capsaicin (cap; 100 nM), a TRPV1 agonist and (D) capsaicin in the presence of SB366791 (5 μM), a TRPV1 antagonist. ATP (10 μM) was used as an internal control representing the expected maximal response. E: Summarized data for Fig 4A–4D. Data are expressed as changes in the raw 340/380 ratio values ± SEM in response to the intervention. *P< 0.05 vs. propofol or capsaicin treated cells. n = 6 separate cover slips of MCAECs were used.