TRIM32-deficiency potentiates <i>Salmonella typhimurium</i>-induced immune responses <i>in vivo</i>.

<p>(A) Effects of TRIM32-deficiency on clearance of <i>Salmonella typhimurium in vivo</i>. Sex- and age-matched <i>Trim32</i><sup><i>+/+</i></sup> and <i>Trim32</i><sup><i>-/-</i></sup> mice were orally infected with <i>Salmonella typhimurium</i>, and bacterial loads were assessed in the indicated tissues 8 days post infection. (B) Effects of TRIM32-deficiency on <i>Salmonella typhimurium</i>-induced lymphocyte activation. Sex- and age-matched <i>Trim32</i><sup><i>+/+</i></sup> and <i>Trim32</i><sup><i>-/-</i></sup> mice were orally infected with <i>Salmonella typhimurium</i>, and viable cell numbers of spleen were counted 6 days post infection. (C) Effects of TRIM32-deficiency on <i>Salmonella typhimurium</i>-induced secretion of inflammatory cytokines. Sex- and age-matched <i>Trim32</i><sup>+/+</sup> and <i>Trim32</i><sup>-/-</sup> mice (n = 3) were orally infected with <i>Salmonella typhimurium</i> for 6 days, followed by measurement of the levels of inflammatory cytokines in the sera. (D) Effects of TRIM32-deficiency on <i>Salmonella typhimurium</i>-induced inflammatory damage of the intestines of mice. Sex- and age-matched <i>Trim32</i><sup>+/+</sup> and <i>Trim32</i><sup>-/-</sup> mice were orally infected with <i>Salmonella typhimurium</i>, and the intestines of mice were used for histological analysis (H&E staining). (E) Effects of TRIM32-deficiency on <i>Salmonella typhimurium</i>-induced loss of body weight. Sex- and age-matched <i>Trim32</i><sup><i>+/+</i></sup> (n = 11) and <i>Trim32</i><sup>−/−</sup> (n = 7) mice were orally infected with <i>Salmonella typhimurium</i>, and their body weight were monitored every day for 7 days. (F) Effects of TRIM32-deficiency on <i>Salmonella typhimurium</i>-induced inflammatory death. Sex- and <i>Trim32</i><sup>+/+</sup> (n = 6) and <i>Trim32</i><sup>-/-</sup> mice (n = 7) were infected with <i>Salmonella typhimurium</i>, and the survival rates of the mice were monitored every day for 20 days. *, p<0.05; **, p<0.01.</p>