Fig 6.TIF (3.16 MB)
TD effect on thapsigargin-induced cytosolic calcium increase in liver cells (n = 3).
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posted on 2016-02-22, 09:39 authored by Enio Rodrigues Vasques, Jose Eduardo Monteiro Cunha, Ana Maria Mendonca Coelho, Sandra N. Sampietre, Rosely Antunes Patzina, Emilio Elias Abdo, Helena B. Nader, Ivarne L. S. Tersariol, Marcelo Andrade Lima, Carlos M. G. Godoy, Tiago Rodrigues, Eleazar Chaib, Luiz A. C. D’AlbuquerquePanel A—Images of 340/380 nm fluorescence ratio using a non-true color scale. Scale bars—50 μm; Time shown in white inside each frame; Panel B—Relative quantification of changes in calcium levels. Arrows indicate additions. THAP—4 μM thapsigargin; IONO—0.25 μM ionomycin; TD—30 μM trisulfated disaccharide.
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Trisulfate Disaccharide Decreases Calcium OverloadBRL 3A hepatic rat cell culturescytosolic calciumintracellular calcium levelsXIPintracellular calciumLiver tissue samplesliver ischemiacalcium extrusionreperfusionMDAincreases hepatic tolerancehepatic histologic injury scoresmitochondrial functionASTProtects Liver InjuryALTSodium calcium exchangerMethods Wistar ratscalcium increase preIVTNFILConclusion TD decreases liver cell damageNCX
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