TCR out-of-frame repertoire sharing in monozygous twins is higher than in unrelated individuals, or than predicted by stochastic models of recombination.
The number of shared out-of-frame alpha TCR clonotypes between all 15 pairs among 6 donors consisting of 3 twin pairs (ordinate) is compared to the model prediction (abscissa). To be able to compare pairs of datasets of different sizes, the sharing number was normalized by the product of the cloneset sizes. The three outstanding red circles represent the twin pairs, while the black circles refer to the 12 pairs of unrelated individuals among the 6 twins. The model prediction is based on a generative stochastic model of VJ recombination [13, 14], inferred separately for each donor to account for differences between individuals. It agrees well with the data from unrelated individuals up to a common multiplicative factor, but systematically underestimates sharing in twins. Error bars show one standard deviation.