Systematic Assignment of the Configuration of Flexible Natural Products by Spectroscopic and Computational Methods:  The Bistramide C Analysis

The combination of NMR NOE, chemical shift, and <i>J</i>-coupling measurements with molar rotation and circular dichroism (CD) determinations, including RI-DFT BP86/aug-cc-pVDZ calculations, reduced a candidate pool of 1024 possible stereoisomers of (+)-bistramide C to a single absolute configuration assignment for the 10 stereogenic carbons of the marine natural product.