Susceptibility of Bisulfite DNA and SafeBis DNA to UNG treatment

Copyright information:

Taken from "Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA"

Nucleic Acids Research 2006;35(1):e4-e4.

Published online 28 Nov 2006

PMCID:PMC1747185.

© 2006 The Author(s)

Detection of methylated DNA with the TMEFF2-HM real-time PCR using 1 ng Bisulfite DNA (open squares), SafeBis DNA (black squares) and 10 000 copies of TMEFF2 PCR product (black triangles). Broken lines indicate no template controls. The PCR products were previously generated with dUTP. Whereas all three different template DNAs are amplified without prior UNG treatment (), only the SafeBis DNA shows amplification after UNG treatment (). The treatment was performed with 0.2 U UNG in closed PCR vessels for 10 min at 37°C.