Following torsional screw extraction analysis, the surface of the threaded portion of removed pedicle screws (PSs) was evaluated by FE-SEM and EDX mapping. Little or no bony tissues were found on the surfaces of the control screws at 1-month (1M) (A) or 3-months post-surgery (3M) (B). A number of bony tissues (white arrows in the magnified pictures) were observed on the surfaces of bioactive screws at 1M (C) and 3M (D) post-surgery. Scale bar: 10 μm.