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Surface Labeling of Enveloped Viruses Assisted by Host Cells
journal contribution
posted on 2012-04-20, 00:00 authored by Bi-Hai Huang, Yi Lin, Zhi-Ling Zhang, Fangfang Zhuan, An-An Liu, Min Xie, Zhi-Quan Tian, Zhenfeng Zhang, Hanzhong Wang, Dai-Wen PangLabeling of virus opens new pathways for the understanding
of viruses
themselves and facilitates the utilization of viruses in modern biology,
medicine, and materials. Based on the characteristic that viruses
hijack their host cellular machineries to survive and reproduce themselves,
a host-cell-assisted strategy is proposed to label enveloped viruses.
By simply feeding Vero cells with commercial 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap
biotinyl) (sodium salt) (Biotin-Cap-PE), we obtained biotinylated
Vero cells whose membrane systems were modified with biotin. Subsequently,
pseudorabies viruses (PrV) were cultivated in the biotinylated Vero
cells, and the PrV progenies were spontaneously labeled with Biotin-Cap-PE
during viral natural assembly process. Since the viral natural assembly
process was employed for the labeling, potential threats of genetic
engineering and difficulties in keeping viral natural bioactivity
were avoided. Importantly, this labeling strategy for enveloped virus
greatly reduces the technical complexity and allows researchers from
different backgrounds to apply it for their specified demands.