bc500008a_si_001.pdf (1.19 MB)
Surface-Chemistry Effect on Cellular Response of Luminescent Plasmonic Silver Nanoparticles
journal contribution
posted on 2015-12-17, 01:05 authored by Shasha Sun, Chen Zhou, Sishan Chen, Jinbin Liu, Jing Yu, Jennifer Chilek, Liang Zhao, Mengxiao Yu, Rodrigo Vinluan, Bo Huang, Jie ZhengCellular response of inorganic nanoparticles
(NPs) is strongly
dependent on their surface chemistry. By taking advantage of robust
single-particle fluorescence and giant Raman enhancements of unique
polycrystalline silver NPs (AgNPs), we quantitatively investigated
effects of two well-known surface chemistries, passive PEGylation
and active c-RGD peptide conjugation, on in vitro behaviors of AgNPs at high temporal and spatial resolution as well
as chemical level using fluorescence and Raman microscopy. The results
show that specific c-RGD peptide−αvβ3 integrin interactions not only induced endosome formation
more rapidly, enhanced constrained diffusion, but also minimized nonspecific
chemical interactions between the NPs and intracellular biomolecules
than passive PEGylation chemistry; as a result, surface enhanced Raman
scattering (SERS) signals of c-RGD peptides were well resolved inside
endosomes in the live cells, while Raman signals of PEGylated AgNPs
remained unresolvable due to interference of surrounding biomolecules,
opening up an opportunity to investigate specific ligand–receptor
interactions in real time at the chemical level.