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Supplementary Table 1. ;Supplementary Table 2. ;Supplementary Table 3. ;Supplementary Table 4 ;Supplementary Figure 1 from In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated pCO2 vent sites

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posted on 2016-11-23, 13:38 authored by Miles Lamare, Michelle Liddy, Sven Uthicke
Carbonate chemistry, temperature and chlorophyll concentration at control and vent sites during Experiment 1 (Table 1A) and Experiment 2 (Table 1B) in Milne Bay, Papua New Guinea. Experiment 1 encompassed two independent trials, while Experiment 2 encompassed four independent trials. For each experiment and trial we report: (i) chemistry measurements based on TA/DIC measurements collected from water samples (N = 2 or 3) during daytime at two consecutive days during each experiments, and (ii) SeaFET mooring data on water temperature and pH are represented as average, minimum and maximum values measured during deployments.;Nested two-way ANOVA for total length (2A) and arm asymmetry (2B) for Echinometra spp. A larvae raised at Dobu control and vent sites at 24 and 48 hours exposure. The fixed factors (pH and time) are nested within the two consecutive experiments.;Nested two-way ANOVA for total length (3A) and arm asymmetry (3B) for 48-h old Echinometra spp. A larvae spawned from either Upa-Upasina vent or control adults and reciprocally transplanted to both Upa-Upasina control and vent sites. The fixed factors (pH and adult source) are nested within the two consecutive experiments.;Nested two-way ANOVA for total length (3A) and arm asymmetry (3B) for 48-h old Echinometra spp. A larvae spawned from either Upa-Upasina vent or control adults and reciprocally transplanted to both Upa-Upasina control and vent sites. The fixed factors (pH and adult source) are nested within the two consecutive experiments.;Seawater pH(T) measurements at control and vent sites for Dobu Island (A) and Upu-Upasina (B). Measurements are made using a seaFET pH/temperature logger that was moored at 2 - 3 m depth.

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    Proceedings of the Royal Society B: Biological Sciences

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