Supplementary Material for: <i>TGFBR3</i> Polymorphisms and Its Haplotypes Associated with Chronic Hepatitis B Virus Infection and Age of Hepatocellular Carcinoma Occurrence

<i>Objective:</i> Hepatocellular carcinoma (HCC) is one of the most common cancers and is mainly caused by viral infections including hepatitis B virus (HBV). Recently, the decreased expression level of the <i>transforming growth factor, beta receptor III</i> (<i>TGFBR3</i>) gene, has been implicated in HCC and other human cancers. This study investigated whether <i>TGFBR3</i> polymorphisms might be associated with HBV clearance and HCC occurrence. <i>Methods:</i> This study identified 27 single nucleotide polymorphisms (SNPs) in the exon, promoter, and exon-intron boundary regions of <i>TGFBR3</i> by resequencing in 24 individuals. Then, 9 SNPs in the promoter and exons of the gene were genotyped from 1,065 Koreans composed of 637 chronic carriers (CC) and 428 spontaneously recovered (SR) subjects. <i>Results:</i> Two SNPs, <i>rs1805113</i> (Phe676Phe) in exon 13 and <i>rs1805117</i> in 3′-UTR (p = 0.009 and p = 0.008, respectively) were significantly associated with HBV clearance. In addition, Cox relative hazards analyses revealed that haplotype<i> BL2_ht2 </i>showed a significant association with the age of HCC occurrence among chronic HBV patients (relative hazard = 1.38; p = 0.007). <i>Conclusion:</i> Our findings suggest that <i>TGFBR3</i> polymorphisms and its haplotypes might be associated with HBV clearance and age of HCC occurrence.