Supplementary Material for: Visualization of Sex-Dimorphic Changes in the Intestinal Transcriptome of <b><i>Fabp2</i></b> Gene-Ablated Mice
2012-05-11T00:00:00Z (GMT) by
<b><i>Background/Aims:</i></b> Sex differences in gene expression program have not been effectively explored at the transcriptome level. We aimed to develop a method for the analysis of transcriptome data to identify sex differences and sex-dimorphic responses to experimental conditions in mice. <b><i>Methods:</i></b> Profiling of the small intestine transcriptome of chow-fed C57BL/6J (wild-type, WT) and <i>Fabp2</i><sup>–/–</sup> mice was carried out by microarray analysis. Sex-specific and androgynous effects of <i>Fabp2</i> gene ablation were examined using FlexArray V1.6 by comparing WT to <i>Fabp2</i><sup>–/–</sup> mice. The data generated were exported into a single spreadsheet, collated and transformed to identify the differentially expressed genes for pathway analysis. <b><i>Results:</i></b> The method revealed enrichment of 17 sex-dimorphic pathways in the small intestine of WT mice compared to only 4 in <i>Fabp2</i><sup>–/–</sup> mice. Comparison of the effects of Fabp2 loss in individual sexes revealed a male-specific upregulation of 5 pathways involved in the production of unsaturated fatty acids, and a female-specific downregulation of pathways involved in xenobiotic metabolism. <b><i>Conclusions:</i></b> Our approach detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms.